BOSSI ELENA | Università degli studi dell'Insubria

E-mail

Elena.Bossi@uninsubria.it

Numero telefonico

0332 42 1318

ELENA BOSSI ZEN
Born in Varese (Italy), November, 16Th., 1967 Married, two kids

Orcid ID orcid.org/0000-0002-9549-2153

EDUCATION

2001, PhD In Physiological Science at University of Milano,

1991 University degree in Biological Science at University of Milano,

ERC Area LS: LS1_8 Biophysics (e.g. transport mechanisms, bioenergetics, fluorescence) LS4_2 Comparative physiology and pathophysiology LS3_2 Cell biology and molecular transport mechanisms

POSITION

2014-today Associate Professor of Physiology Department of Biotechnology and Life Sciences University of Insubria, Principal Investigator of the Laboratory of Cellular and Molecular Physiology
2000-2014 Research associate- Department of Biotechnology and life sciences, University of Insubria, Laboratory of Cellular and Molecular Physiology .

SCIENTIFIC EXPERIENCE

Structure and function studies of membrane protein and membrane transporters, interaction of Nanoparticle with membrane, using electrophysiological, fluorometric, biochemical, and molecular biology tools, in model systems (Xenopus oocytes and HEK cell).
1998-2000 PhD student, Department of Structural and Functional Biology, University of Milan (now University of Insubria), Laboratory of Cellular and Molecular Physiology, Structure and function of cotrasporters.
09-12/1999 Institute of Physiology University of Zurich. Structure and function studies on Na cotransporter Na/Pi type IIb by SCAM (substitute cysteine accessibility method), electrophysiology and radio-labelled methods.
1993-1998 Technical research assistant- Department of Structural and Functional Biology, University of Milan (now University of Insubria), Laboratory of Cellular and Molecular Physiology. Properties and regulation of membrane channels, transport systems and receptors (identification, relationships and role of subunits) using electrophysiological, imunochemical, biochemical, and recombinant DNA techniques, in model systems (Xenopus oocytes) and in vitro.
02-03/1997 Department of Physiology and Pharmacology, Sackler School of Medicine, Tel Aviv University Laboratory of Prof. Dascal: biochemical analysis of expression of voltage dependent calcium channels: identification of relationship and role of Gs protein a subunits.
08-09/1995 CNR-NCRD Fellowship in the Department of Physiology and Pharmacology, Sackler School of Medicine, Tel Aviv University, Laboratory of Prof. Dascal (Joint Project CNR-NCRD): use of molecular biology techniques in studies about heterogeneity of voltage dependent calcium channels for identification and role of subunits.
1990-1992 ECC, JRC, Environmental Institute, Life Science Division in the laboratory of Dr. E. Marafante in collaboration with IAEA, Laboratory of marine Radioactivity, Monaco. Use of primary fish cell culture for studying effects of exposition to xenobiotics on the DNA. Development of method to identify DNA's adducts with HPLC-32P-Post Labeling, and other exposition parameters to genotoxic substances

COLLABORATION

-Giovanni Bernardini-Rosalba Gornati University of Insubria
-Loredano Pollegioni- Silvia Sacchi University of Insubria
-Marco Saroglia-Genciana Terova University of Insubria
-Daniela Negrini-Andrea Moriondo University of Insubria
-Dr. Michela Castagna University of Milan.
-Salvatore Bozzaro University of Turin.
-Gabor Kottra Molecular Technische Universitat Munchen, Germany
-Tiziano Verri Università del Salento(LE)
-Carmen Aragon and Beatriz Lopez Corcuera-Universidad Autonoma de Madrid- Spain
-Spanevello - Dr Francesca Cherubino Fondazione Maugeri IRCCS, Tradate (VA), Italy
-Ian Forster University of Zurich,Switzerland
-Natan Dascal University of Tel Aviv, Israel
-Ivar Ronnestad Department of Biology, University of Bergen, Bergen 5020, Norway
-Stefan Broer Research School of Biology, The Australian National University, Canberra,Australia

Ricerca

Dal 1994 a oggi, ha svolto attività di ricerca presso il Laboratorio di Fisiologia Cellulare e Molecolare occupandosi prevalentemente di Studi struttura- funzione e di regolazione di canali ionici e trasportatori di membrana in sistemi per espressione eterologa (oociti di Xenopus laevis e linee cellulari). Mediante l’utilizzo di tecniche di biologia molecolare, tecniche elettrofisiologiche, immunochimiche e biochimiche sono state caratterizzate numerose proteine coinvolte nella traslocazione di ioni e soluti, dal 1997 al 2001 l’attività si è concentrata sulla modulazione di un canale del calcio cardiaco (di tipo L) e di un canale del potassio (IRK1) e sulla caratterizzazione di alcune correnti endogene degli oociti di Xenopus. Dal 1998 la ricerca si è focalizzata principalmente sui trasportatori di membrana elettrogenici, inizialmente KAAT1 (trasportatore intestinale di amino acidi neutri di Manduca sexta) per poi estendersi ai trasportatori di neurotrasmettitori sempre appartenenti alla famiglia degli SLC6a in particolare su GAT1, e GLYT 1 e 2. Dal 2008 le indagini hanno coinvolto un’altra importante famiglia di trasportatori di nutrienti, i trasportatori di oligo- peptidi PepT1 (SLC15a) e dal 2011 si sono aggiunti trasportatori di aminoacidi neutri di mammifero e trasportatori di ioni bivalenti. Più recentemente la caratterizzazione del trasporto di ferro e altri metalli divalenti, ha portato a ricerche sull’interazione tra Nanoparticelle (NP) metalliche e membrana plasmatica.

Gli studi utilizzano di tecniche elettrofisiologiche, immunochimiche, biochimiche, fluorimetriche, tecniche di biologia molecolare. Tutti gli studi sono compiuti su proteine eterologamente espresse in oociti di Xenopus o su linee cellulari. La caratterizzazione funzionale, componente primaria della ricerca è condotta principalmente tramite analisi elettrofisiologiche.

Membro del centro di neuroscienze di Ateneo e del centro Protein factory (Insubria –Politecnico di Milano)

My research work is done in laboratory of cellular and molecular physiology, my research is about proteins involved in translocation of amino acids and small peptides across the cellular membrane. The work is focused on the structure-function relationships in amino acids, peptides and neurotransmitter cotransporters and on investigation of the role of ions in translocation. We use heterologous expression in Xenopus oocytes, coupled to electrophysiology and fluorophores in an attempt to identify specific aspects of the transport function. The solut carriers of different family (SLC1A, SLC6A, SLC15A,SLC11A) togheter with the interaction of nanoparticles with tehe membrane are at present our main objects of my study. The techniques used include microinjection, voltage-clamp, site-specific mutagenesis, uptake measurements and immunocytochemistry, single oocyte chemiluminescence.

Portale IRInSubria

Pubblicazioni in evidenza

1. NEGRINI D, MARCOZZI C, SOLARI E, BOSSI E, CINQUETTI R, REGUZZONI M, MORIONDO A. Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in peripheral diaphragmatic lymphatics. Am J Physiol Heart Circ Physiol. 2016 Aug 5: doi: 10.1152/ajpheart.00193.2016. [Epub ahead of print] PMID:27496876

2. MARGHERITIS E, IMPERIALI FG, CINQUETTI R, VOLLERO A, TEROVA G, RIMOLDI S, GIRARDELLO R, BOSSI E (2016) Amino acid transporter BAT1 (slc6a19) and ancillary protein: impact on function. Pflugers Archiv : European journal of physiology. 468(8):1363-74, Doi 10.1007/s00424-016-1842-5

3. BOSSI E, ZANELLA D, GORNATI R, BERNARDINI G (2016) Cobalt oxide nanoparticles can enter inside the cells by crossing plasma membranes. Scientific reports 6: 22254 Doi 10.1038/srep22254 PMID: 26924527

4. SIGNORETTO E, ZIERLEA J, BISSINGERA R, CASTAGNA M, BOSSI E, LANGA F. (2016) Triggering of Suicidal Erythrocyte Death by Pazopanib. Cell Physiol Biochem. 2016;38(3):926-38. doi: 10.1159/000443045. Epub 2016 Mar 4.PMID: 26937949

5. VOLLERO A, IMPERIALI FG, CINQUETTI R, MARGHERITIS E, PERES A & BOSSI E. (2016). The D-amino acid transport by the invertebrate SLC6 transporters KAAT1 and CAATCH1 from Manduca sexta. Physiological reports 4(4). pii: e12691. doi: 10.14814/phy2.12691 PMID: 26884475

6. BURACCO S, PERACINO B, CINQUETTI R, SIGNORETTO E, VOLLERO A, IMPERIALI F, CASTAGNA M, BOSSI E, BOZZARO S. Dictyostelium Nramp1, structurally and functionally close to mammalian DMT1 transporter, mediates phagosomal iron efflux. J Cell Sci. 2015 Sep 1;128(17):3304-16. pii: jcs.173153. doi: 10.1242/jcs.173153 PMID: 26208637.

7. RIMOLDI S, BOSSI E, HARPAZ S, CATTANEO AG, BERNARDINI G, SAROGLIA M, TEROVA G. Intestinal B(0)AT1 (SLC6A19) and PEPT1 (SLC15A1) mRNA levels in European sea bass (Dicentrarchus labrax) reared in fresh water and fed fish and plant protein sources. J Nutr Sci. 2015 May 20;4:e21. doi:10.1017/jns.2015.9. eCollection2015. PubMed PMID: 26097704; PubMed Central PMCID: PMC4462763.

8. KWASEK K, TEROVA G, LEE BJ, BOSSI E, SAROGLIA M & DABROWSKI K. (2014). Dietary methionine supplementation alters the expression of genes involved in methionine metabolism in salmonids. Aquaculture 433, 223-228. 10.1016/j.aquaculture.2014.05.031

9. MARGHERITIS E, TEROVA G, CINQUETTI R, PERES A, BOSSI E. Functional properties of a newly cloned fish ortholog of the neutral amino acid transporter B(0)AT1 (SLC6A19). Comp Biochem Physiol A Mol Integr Physiol. 2013 Jul 1. doi:pii:S1095-6433(13)00170-0. 10.1016/j.cbpa.2013.06.027. [Epub ahead of print] PubMedPMID: 23827520.

10. PERES A, VOLLERO A, MARGHERITIS E, D'ANTONI F, BOSSI E. An Inverse Relationship Links Temperature and Substrate Apparent Affinity in the Ion-Coupled Cotransporters rGAT1 and KAAT1. Int J Mol Sci. 2012 Nov 22;13(12):15565-74. doi: 10.3390/ijms131215565. PubMed PMID: 23443081; PubMed Central PMCID: PMC3546649.

11. MARGHERITIS E, TEROVA G, OYADEYI AS, RENNA MD, CINQUETTI R, PERES A, BOSSI E. Characterization of the transport of lysine-containing dipeptides by PepT1 orthologs expressed in Xenopus laevis oocytes. Comp Biochem Physiol A Mol Integr Physiol. 2013 Mar;164(3):520-8. doi: 10.1016/j.cbpa.2012.12.016. Epub 2012 Dec 23. PubMed PMID: 23268205.

12. BOSSI E, CHERUBINO F, MARGHERITIS E, OYADEYI AS, VOLLERO A, PERES A. Temperature effects on the kinetic properties of the rabbit intestinal oligopeptide cotransporter PepT1 Pflugers Arch. 2012 Aug;464(2):183-91. Epub 2012 Jun 23. PMID:22729751

13. CHERUBINO F, BERTRAM S, BOSSI E, PERES A. Role of external chloride in the presteady state and reverse transport current in the GABA transporter GAT1. 2012 Am J Physiol Cell Physiol. Am J Physiol Cell Physiol. 2012 Apr;302(8):C1096-108. Epub 2011 Dec 14. PMID:22173867

14. GIOVANOLA M, D’ANTONI F, SANTACROCE M, MARI SA, CHERUBINO F, BOSSI E, SACCHI VF AND CASTAGNA M Role of a conserved glycine triplet in the NSS amino acid transporter KAAT1, Biochim Biophys Acta. 2012 Feb 28;1818(7):1737-1744. PMID:22402268

15. PÉREZ-SILES G, NÚÑEZ E, MORREALE A, LEO-MACÍAS A, PITA G, CHERUBINO F, SANGALETTI R, BOSSI E, ORTÍZ AR, ARAGÓN C AND LÓPEZ-CORCUERA B. An external vestibule aspartate of glycine transporter 2 (GLYT2) controls cation access and transport coupling Biochem J. 2012 Mar 1;442(2):323-34. PubMed PMID: 22132725.

16. BERTRAM S, CHERUBINO F, BOSSI E, CASTAGNA M, PERES A. GABA reverse transport by the neuronal cotransporter GAT1: influence of internal chloride depletion. Am J Physiol Cell Physiol. 2011 Nov;301(5):C1064-73. Epub 2011 Jul 20. PubMed PMID: 21775701.

17. RENNA M., OYADEYI A.S., BOSSI E., KOTTRA G., PERES A. Functional and structural determinants of reverse operation in the pH dependent oligopeptide transporter PepT1. Cell Mol Life Sci. 2011 Sep;68(17):2961-75. Epub 2010 Dec 23. PubMed PMID:21181229.

18. BOSSI E., RENNA M., SANGALETTI R., D’ANTONI F., CHERUBINO F. KOTTRA G., PERES A. Residues R282 and D341 act as elctrostatic gates in the proton-dependent oligopeptide transporter rbPepT1 J Physiol. 2011 Feb 1;589(Pt 3):495-510. Epub 2010 Nov 29. PubMed PMID: 21115649; PubMed Central PMCID: PMC3055539.

19. RENNA M., SANGALETTI R., BOSSI E., CHERUBINO F. KOTTRA G., PERES A. unified modelling of the mammalian and fish proton-dependent oligopeptide transporter PepT1,. Channels. 2011 2011 Jan 1;5(1):89-99. Epub 2011 Jan 1. PMID:20953145

20. CHERUBINO F., MISZNER A., RENNA M., SANGALETTI R., GIOVANNARDI S., AND BOSSI E.- GABA transporter lysine 448: a key residue for Tricyclic Antidepressants interaction- Cell Mol Life Sci (2009) Dec; 66(23):3797-808. PMID: 19756379.

21. CHERUBINO F., BOSSI E., MISZNER A., GHEZZI C., PERES A.- Transient currents in the glycine cotransporter GLYt1 reveal different steps in transport mechanism- J Mol

Neurosci. 2010 Jun;41(2):243-51. Epub 2009 Aug 27. PubMed PMID: 19711201.

22. SANGALETTI R, TEROVA G, PERES A, BOSSI E, CORÀ S, SAROGLIA M. Functional expression of the oligopeptide transporter PepT1 from the sea bass (Dicentrarchus labrax). Pflugers Arch. 2009 Nov;459(1):47-54. Epub 2009 Jul 18. PMID:19618206.

23. CASTAGNA M., BOSSI E, AND SACCHI V.F., Molecular physiology of the insect amino acid transporters KAAT1 and CAATCH1 in the light of the structure of the homologous protein LeuT. Review- Insect molecular biology (2009). Jun;18(3):265-79. Epub 2009 Apr 6. PMID: 19389142

24. BETTÈ S, CASTAGNA M, BOSSI E, PERES A, SACCHI VF. The SLC6/NSS family members KAAT1 and CAATCH1 have weak chloride dependence. Channels (Austin). 2008 Sep 15;2(5). PMID: 19066444

25. GRIMALDI A, TETTAMANTI G, ACQUATI F, BOSSI E, GUIDALI ML, BANFI S, MONTI L, VALVASSORI R, DE EGUILEOR M. A hedgehog homolog is involved in muscle formation and organization of Sepia officinalis (mollusca) mantle. Dev Dyn. 2008 Mar;237(3):659-71. PMID: 18265019

26. BOSSI E, FABBRINI MS, CERIOTTI A. Exogenous protein expression in Xenopus oocytes: basic procedures. Methods Mol Biol. 2007;375:107-31. Review. PMID: 17634599

27. MISZNER A, PERES A, CASTAGNA M, BETTÈ S, GIOVANNARDI S, CHERUBINO F, BOSSI E. Structural and functional basis of amino acid specificity in the invertebrate cotransporter KAAT1. J Physiol. 2007 Jun 15;581(Pt 3):899-913. Epub 2007 Apr 5. PMID: 17412764

28. FORSTER IC, VIRKKI L, BOSSI E, MURER H, BIBER J. Electrogenic kinetics of a mammalian intestinal type IIb Na(+)/P(i) cotransporter. J Membr Biol. 2006;212(3):177-90. Epub 2007 Mar 6. PMID: 17342377

29. BOSSI E, SORAGNA A, MISZNER A, GIOVANNARDI S, FRANGIONE V, PERES A. Oligomeric structure of the neutral amino acid transporters KAAT1 and CAATCH1. Am J Physiol Cell Physiol. 2007 Apr;292(4):C1379-87. Epub 2006 Nov 29. PMID: 17135296

30. MARI SA, SORAGNA A, CASTAGNA M, SANTACROCE M, PEREGO C, BOSSI E, PERES A AND SACCHI V F. (2006) Role of the Conserved Glutamine 291 in the Rat Gamma-Aminobutyric Acid Transporter RGAT-1. Cell Mol Life Sci 63: pp 100-111. PMID: 16378241

31. SORAGNA A, BOSSI E, GIOVANNARDI S, PISANI R AND PERES A (2005) Functionally Independent Subunits in the Oligomeric Structure of the GABA Cotransporter rGAT1. Cell Mol Life Sci 62: pp 2877-2885. PMID: 16314925

32. SORAGNA A, BOSSI E, GIOVANNARDI S, PISANI R AND PERES A (2005) Relations Between Substrate Affinities and Charge Equilibration Rates in the Rat GABA Cotransporter GAT1. J Physiol 562: pp 333-345. PMID: 15513937

33. MARI SA, SORAGNA A, CASTAGNA M, BOSSI E, PERES A AND SACCHI VF (2004) Aspartate 338 Contributes to the Cationic Specificity and to Driver-Amino Acid Coupling in the Insect Cotransporter KAAT1. Cell Mol Life Sci 61: pp 243-256. PMID: 14745502

34. SORAGNA A, MARI S A, PISANI R, PERES A, CASTAGNA M, SACCHI V F AND BOSSI E (2004) Structural Domains Involved in Substrate Selectivity in Two Neutral Amino Acid Transporters. Am J Physiol Cell Physiol 287: pp C754-C761. PMID: 15140745

35. PERES A, GIOVANNARDI S, BOSSI E AND FESCE R (2004) Electrophysiological Insights into the Mechanism of Ion-Coupled Cotransporters. News Physiol Sci 19: pp 80-84. PMID: 15016908

36. SACCHI VF, CASTAGNA M, MARI S A, PEREGO C, BOSSI E AND PERES A (2003) Glutamate 59 Is Critical for Transport Function of the Amino Acid Cotransporter KAAT1. Am J Physiol Cell Physiol 285: pp C623-C632. PMID: 12736138

37. GIOVANNARDI S, FESCE R, BOSSI E, BINDA F AND PERES A (2003) Cl- Affects the Function of the GABA Cotransporter RGAT1 but Preserves the Mutal Relationship Between Transient and Transport Currents. Cell Mol Life Sci 60: pp 550-556. PMID: 12737314

38. BOSSI E, GIOVANNARDI S, BINDA F, FORLANI G AND PERES A (2002) Role of Anion-Cation Interactions on the Pre-Steady-State Currents of the Rat Na(+)-Cl(-)-Dependent GABA Cotransporter rGAT1. J Physiol 541: pp 343-350. PMID: 12042343

39. BINDA F, BOSSI E, GIOVANNARDI S, FORLANI G AND PERES A (2002) Temperature Effects on the Presteady-State and Transport-Associated Currents of GABA Cotransporter RGAT1. FEBS Lett 512: pp 303-307. PMID: 11852100

40. FESCE R, GIOVANNARDI S, BINDA F, BOSSI E AND PERES A (2002) The Relation Between Charge Movement and Transport-Associated Currents in the Rat GABA Cotransporter RGAT1. J Physiol 545: pp 739-750. PMID: 12482883

41. GIOVANNARDI S, FORLANI G, BALESTRINI M, BOSSI E, TONINI R, STURANI E, PERES A AND ZIPPEL R (2002) Modulation of the Inward Rectifier Potassium Channel IRK1 by the Ras Signaling Pathway. J Biol Chem 277: pp 12158-12163. PMID: 11809752

42. FORLANI G, BOSSI E, GHIRARDELLI R, GIOVANNARDI S, BINDA F, BONADIMAN L, IELMINI L AND PERES A (2001) Mutation K448E in the External Loop 5 of Rat GABA Transporter RGAT1 Induces PH Sensitivity and Alters Substrate Interactions. J Physiol 536: pp 479-494. PMID: 11600683

43. FORLANI G, BOSSI E, PEREGO C, GIOVANNARDI S AND PERES A (2001) Three Kinds of Currents in the Canine Betaine-GABA Transporter BGT-1 Expressed in Xenopus Laevis Oocytes. Biochim Biophys Acta 1538: pp 172-180. PMID: 11336788

44. BOSSI E, VINCENTI S, SACCHI V F AND PERES A (2000) Simultaneous Measurements of Ionic Currents and Leucine Uptake at the Amino Acid Cotransporter KAAT1 Expressed in Xenopus Laevis Oocytes. Biochim Biophys Acta 1495: pp 34-39. PMID: 10634930

45. PERES A AND BOSSI E (2000) Effects of pH on the Uncoupled, Coupled and Pre-Steady-State Currents at the Amino Acid Transporter KAAT1 Expressed in Xenopus Oocytes. J Physiol 525 Pt 1: pp 83-89. PMID: 10811727

46. BLUMENSTEIN Y, IVANINA T, SHISTIK E, BOSSI E, PERES A AND DASCAL N (1999) Regulation of Cardiac L-Type Ca2+ Channel by Coexpression of G (Alpha S) in Xenopus Oocytes. FEBS Lett 444: pp 78-84. PMID: 10037152

47. BOSSI E, CENTINAIO E, CASTAGNA M, GIOVANNARDI S, VINCENTI S, SACCHI V F AND PERES A (1999) Ion Binding and Permeation Through the Lepidopteran Amino Acid Transporter KAAT1 Expressed in Xenopus Oocytes. J Physiol 515 ( Pt 3): pp 729-742. PMID: 10066900

48. BOSSI E, SACCHI V F AND PERES A (1999) Ionic Selectivity of the Coupled and Uncoupled Currents Carried by the Amino Acid Transporter KAAT1. Pflugers Arch 438: pp 788-796. PMID: 10591067

49. BOSSI E, CENTINAIO E, MORIONDO A AND PERES A (1998) Ca2+-Dependence of the Depolarization-Inducible Na+ Current of Xenopus Oocytes. J Cell Physiol 174: pp 154-159. PMID: 9428801

50. CENTINAIO E, BOSSI E AND PERES A (1997) Properties of the Ca(2+)-Activated Cl- Current of Xenopus Oocytes. Cell Mol Life Sci 53: pp 604-610. PMID: 9284963