PASSI ALBERTO GIUSEPPE | Università degli studi dell'Insubria

E-mail

[email protected]

Numero telefonico

0332 39 7142,0332 39 7150

CURRICULUM VITAE

ALBERTO PASSI MD PhD

Full Professor of Biochemistry

Department of Medicine and and Surgery (DMC)

School of Medicine - University of Insubria – Varese-ITALY

Via Dunant,5 - 21100 Varese- ITALY. tel. +39+0332+217142 fax. +39+0332+217119

e-mail: [email protected]

Place and Birthday: Cremona, 18.3.1962

EDUCATION

1995 Specialization in Clinical Biochemistry cum laude, University of Pavia.

1992 Doctoral Degree Doctoral Degree in Biochemistry, Department of Biochemistry, University of Pavia

and Genoa, Italy.

1987 Degree in Medicine and Surgery cum laude, University of Pavia, Italy.

ACADEMIC APPOINTMENTS

(2011-present) Full Professor of biochemistry, DSCM, University of Insubria, Varese, School of Medicine

(2008-2011) Full Professor of biochemistry, DSBSC, University of Insubria, Varese, School of Medicine

(2001-2007) Associate Professor, DSBSC, University of Insubria, Varese, School of Medicine

(1998-2001) Assistant Professor, DSBSC, University of Insubria, Varese, School of Medicine.

(1992-1998) Assistant Professor, Department of Biochemistry, University of Pavia, School of Medicine.

(1990-1998) Instructor of Biochemistry and Applied Biochemistry, University of Pavia School of Medicine.

(1998-present) Instructor of Biochemistry and Applied Biochemistry, University of Pavia School of

Medicine.

EXPERIENCE

Research Scholar Cleveland Clinic Foundation (OH– USA) (2000-2001)

Postdoctoral fellow Department of Biochemistry University of Pavia (1991-1992)

SOCIETIES

Italian Biochemistry Society (1990-present)

Italian Connective Tissue Society (1990-present).

International Society of Matrix Biology (1998-present)

American Society for Matrix Biology (2003-present)

American Society of Biochemistry and Molecular Biology (2006-present)

International Society for Hyaluronan Sciences (ISHAS) – board of trustees

AWARDS

Hellenic Biochemistry Society Award (2009)

National Fellowship Biochemical Society (University of Manchester-1990)

Alumnus of Collegio Ghislieri, Pavia, (1981-1987)

BOARD CERTIFICATION AND LICENSURE

1988, Italian Medical Association.

ACADEMIC RESPONSABILITIES

Dean of Sport Sciences Faculty (2008-16) (University of Insubria)

Director of Biotechnology Centre for Human Health (2009-15) (University of Insubria)

Director of Interdepartmental Centre for Applied Sport Sciences (CRISMA) (2016-present) (University of Insubria)

Scientific advisor of Greek Minister of University and Religious Affairs

Component of the “Scientific committee of Regional Sport School-CONI” (Milano)

Scientific advisor of Patras University, Heraklion University, Kapodistrian University (Athens).

Component elected of the National Board of Biochemical professors (2017-19)

JOURNAL EDITORIAL BOARD MEMBERSHIPS

- Editor Journal of Biological Chemistry (2011-2016)

- Associate Editor Connective Tissue Research

- Academic editor Plos ONE

- Editor Matrix Biology

- Editor Open Biology Journal

- Editor of Frontiers in membrane physiology and biophysics

INTERNATIONAL MEETING ATTENDANCE in the last 5 years

Experimental Biology Meeting EB 2012, San Diego (USA)
Brazilian Society of Biochemistry meeting –SBBq Foz Uguazù (Brazil) 2012 (Invited speaker)
EB 2013 in Glycobiology workshop Boston (MA USA) (selected speaker)
Hyaluronan 2013 Oklahoma City (OK – USA) (invited speaker)
Proteoglycans 2013 Intl. Meeting (Frankfurt – Germany) (invited speaker)
FEBS Selected Course, Matrix Pathobiology, Kos (Greece) 2013 (invited speaker)
Symposium “Targeting Hyaluronan in Pathophysiology and Disease” Duesseldorf (Germany) 2014 (invited speaker)
Gordon Conference on Proteoglycans, Andover NH USA, 2014 (invited speaker)

Hyaluronan 2015, Florence 2015 (organizer)
9th International Conference on Proteoglycans and 10th Pan-Pacific Connective Tissue Societies Symposium, Seoul (South Korea) 2015
XXIII Glyko meeting Split (Croatia) 2015 (invited speaker)
5th FEBS Advanced Lecture Course Matrix Pathobiology, Signaling and Molecular Targets

Rhodes (Greece) 2015, (invited speaker)

2nd MBE European Matrix biology Meeting Athens (Greece) 2016 (Invited speaker)
Gordon Conference on Proteoglycans Andover NH (USA) 2016 (selected speaker)
6th FEBS Advanced Lecture Course Matrix Pathobiology, Signaling and Molecular Targets

Spetses (Greece) 2017, (invited speaker)

Hyaluronan 2017 – Cleveland OH (USA) (invited speaker)

BIBLIOGRAPHY

A. Passi is author of 108 peer reviewed papers (Scopus Scholar) more than 60 international meetings presenting about 50 talks. His h-index is 29 (Scopus Scholar 108 pubblications) or 35 (Google scholar with 183 publications). He also gave several seminars in international institutions as University of Bern (Switzerland), NY University (USA), University of Harvard (USA), Cleveland Clinic Foundation (USA), Open University (UK), University of Kuopio (Finland), Uppsala University (Sweden), Patras University (Greece), Muenster University (Germany), Heinrich Heine University Duesseldorf (Germany), University of Warsaw (Poland).

Ricerca

1-STUDI SUGLI ENZIMI COINVOLTI NELLA SINTESI DEI PRECURSORI DEI COMPONENTI DELLA MATRICE EXTRACELLULARE
Nelle prime esperienze di ricerca, culminate col lavoro di tesi di laurea, il prof. Passi si è occupato delle caratteristiche cinetiche dell?enzima UDP-glucoso deidrogenasi. L?attività di tale enzima che catalizza la sintesi dei precursori degli acidi uronici, è fondamentale nella sintesi dell?acido ialuronico e dei glicosaminoglicani (GAG), catene polisaccaridiche dei proteoglicani (PG).
Gli esperimenti attuali che sono in corso vedono come obiettivo l?uso della UDPG deidrogenasi da Xenopus laevis recentemente clonata nel nostro laboratorio in vari modelli. L?obiettivo di tali esperimenti, oltre alla caratterizzazione cinetica e strutturale di tale proteina, è quello di osservare l?effetto dell?attività dell?enzima UDPG deidrogenasi sulla quantità dei precursori citoplasmatici (UDP zuccheri) e di conseguenza sulla sintesi di altri enzimi coinvolti nella sintesi dei glicosaminoglicani, questo ultimo aspetto tramite studi di gene expression.
2-STUDI SULLE MODIFICAZIONI DELLA MATRICE EXTRACELLULARE IN MODELLI CELLULARI IN VITRO
La senescenza cellulare è descritta in molte cellule eucariotiche, ed è costituito dalla progressiva perdita della capacità proliferativa delle cellule col passare delle colture. Attualmente questa linea di ricerca si è maggiormente concentrata sulla senescenza di cellule vascolari umane, cioè endoteli e muscolari lisce. Mediante studi di gene expression si è valutata anche in questo caso l?alterazione della matrice extracellulare, con un significativo aumento della sintesi di ialuronico e osteopontina nelle muscolari lisce.
3-STUDI SULLE MODIFICAZIONI DELLA MATRICE EXTRACELLULARE IN MODELLI IN VIVO.
Un ulteriore aspetto della nostra ricerca riguardante la degenerazione tessutale è lo studio della degradazione della matrice extracellulare durante la formazione dell?edema polmonare acuto indotto con diversi meccanismi. In

Portale IRInSubria

Pubblicazioni in evidenza

1: Vigetti D, Karousou E, Viola M, Deleonibus S, De Luca G, Passi A. Hyaluronan:
Biosynthesis and signaling. Biochim Biophys Acta. 2014 Feb 7. pii:
S0304-4165(14)00047-6. doi: 10.1016/j.bbagen.2014.02.001. [Epub ahead of print]
Review. PubMed PMID: 24513306.

2: Hascall VC, Wang A, Tammi M, Oikari S, Tammi R, Passi A, Vigetti D, Hanson RW,
Hart GW. The dynamic metabolism of hyaluronan regulates the cytosolic
concentration of UDP-GlcNAc. Matrix Biol. 2014 Jan 30. pii:
S0945-053X(14)00035-3. doi: 10.1016/j.matbio.2014.01.014. [Epub ahead of print]
PubMed PMID: 24486448.

3: Vigetti D, Viola M, Karousou E, De Luca G, Passi A. Metabolic control of
hyaluronan synthases. Matrix Biol. 2013 Oct 14. pii: S0945-053X(13)00131-5. doi:
10.1016/j.matbio.2013.10.002. [Epub ahead of print] PubMed PMID: 24134926.

4: Viola M, Bartolini B, Vigetti D, Karousou E, Moretto P, Deleonibus S, Sawamura
T, Wight TN, Hascall VC, De Luca G, Passi A. Oxidized low density lipoprotein
(LDL) affects hyaluronan synthesis in human aortic smooth muscle cells. J Biol
Chem. 2013 Oct 11;288(41):29595-603. doi: 10.1074/jbc.M113.508341. Epub 2013 Aug
26. PubMed PMID: 23979132; PubMed Central PMCID: PMC3795257.

5: Motolese A, Vignati F, Brambilla R, Cerati M, Passi A. Interaction between a
regenerative matrix and wound bed in nonhealing ulcers: results with 16 cases.
Biomed Res Int. 2013;2013:849321. doi: 10.1155/2013/849321. Epub 2013 Jul 18.
PubMed PMID: 23971047; PubMed Central PMCID: PMC3732600.

6: Karousou E, Stachtea X, Moretto P, Viola M, Vigetti D, D'Angelo ML, Raio L,
Ghezzi F, Pallotti F, De Luca G, Karamanos NK, Passi A. New insights into the
pathobiology of Down syndrome--hyaluronan synthase-2 overexpression is regulated
by collagen VI α2 chain. FEBS J. 2013 May;280(10):2418-30. doi:
10.1111/febs.12220. Epub 2013 Mar 28. PubMed PMID: 23452080.

7: Vigetti D, Deleonibus S, Moretto P, Karousou E, Viola M, Bartolini B, Hascall
VC, Tammi M, De Luca G, Passi A. Role of UDP-N-acetylglucosamine (GlcNAc) and
O-GlcNAcylation of hyaluronan synthase 2 in the control of chondroitin sulfate
and hyaluronan synthesis. J Biol Chem. 2012 Oct 12;287(42):35544-55. doi:
10.1074/jbc.M112.402347. Epub 2012 Aug 10. PubMed PMID: 22887999; PubMed Central
PMCID: PMC3471761.

8: Moriondo A, Marcozzi C, Bianchin F, Reguzzoni M, Severgnini P, Protasoni M,
Raspanti M, Passi A, Pelosi P, Negrini D. Impact of mechanical ventilation and
fluid load on pulmonary glycosaminoglycans. Respir Physiol Neurobiol. 2012 May
31;181(3):308-20. doi: 10.1016/j.resp.2012.03.013. Epub 2012 Mar 29. PubMed PMID:
22484819.

9: Piccioni F, Malvicini M, Garcia MG, Rodriguez A, Atorrasagasti C, Kippes N,
Piedra Buena IT, Rizzo MM, Bayo J, Aquino J, Viola M, Passi A, Alaniz L,
Mazzolini G. Antitumor effects of hyaluronic acid inhibitor 4-methylumbelliferone
in an orthotopic hepatocellular carcinoma model in mice. Glycobiology. 2012
Mar;22(3):400-10. doi: 10.1093/glycob/cwr158. Epub 2011 Oct 28. PubMed PMID:
22038477.

10: Vigetti D, Rizzi M, Moretto P, Deleonibus S, Dreyfuss JM, Karousou E, Viola
M, Clerici M, Hascall VC, Ramoni MF, De Luca G, Passi A. Glycosaminoglycans and
glucose prevent apoptosis in 4-methylumbelliferone-treated human aortic smooth
muscle cells. J Biol Chem. 2011 Oct 7;286(40):34497-503. doi:
10.1074/jbc.M111.266312. Epub 2011 Jul 18. PubMed PMID: 21768115; PubMed Central
PMCID: PMC3186361.

11: Moriondo A, Marcozzi C, Bianchin F, Passi A, Boschetti F, Lattanzio S,
Severgnini P, Pelosi P, Negrini D. Impact of respiratory pattern on lung
mechanics and interstitial proteoglycans in spontaneously breathing anaesthetized
healthy rats. Acta Physiol (Oxf). 2011 Oct;203(2):331-41. doi:
10.1111/j.1748-1716.2011.02317.x. Epub 2011 May 28. PubMed PMID: 21518268.

12: Merrilees MJ, Beaumont BW, Braun KR, Thomas AC, Kang I, Hinek A, Passi A,
Wight TN. Neointima formed by arterial smooth muscle cells expressing versican
variant V3 is resistant to lipid and macrophage accumulation. Arterioscler Thromb
Vasc Biol. 2011 Jun;31(6):1309-16. doi: 10.1161/ATVBAHA.111.225573. Epub 2011 Mar
24. PubMed PMID: 21441139; PubMed Central PMCID: PMC3123728.

13: Tammi RH, Passi AG, Rilla K, Karousou E, Vigetti D, Makkonen K, Tammi MI.
Transcriptional and post-translational regulation of hyaluronan synthesis. FEBS
J. 2011 May;278(9):1419-28. doi: 10.1111/j.1742-4658.2011.08070.x. Epub 2011 Mar
25. Review. PubMed PMID: 21362137.

14: Vigetti D, Clerici M, Deleonibus S, Karousou E, Viola M, Moretto P, Heldin P,
Hascall VC, De Luca G, Passi A. Hyaluronan synthesis is inhibited by adenosine
monophosphate-activated protein kinase through the regulation of HAS2 activity in
human aortic smooth muscle cells. J Biol Chem. 2011 Mar 11;286(10):7917-24. doi:
10.1074/jbc.M110.193656. Epub 2011 Jan 12. PubMed PMID: 21228273; PubMed Central
PMCID: PMC3048678.

15: Acquati F, Bertilaccio S, Grimaldi A, Monti L, Cinquetti R, Bonetti P, Lualdi
M, Vidalino L, Fabbri M, Sacco MG, van Rooijen N, Campomenosi P, Vigetti D, Passi
A, Riva C, Capella C, Sanvito F, Doglioni C, Gribaldo L, Macchi P, Sica A, Noonan
DM, Ghia P, Taramelli R. Microenvironmental control of malignancy exerted by
RNASET2, a widely conserved extracellular RNase. Proc Natl Acad Sci U S A. 2011
Jan 18;108(3):1104-9. doi: 10.1073/pnas.1013746108. Epub 2010 Dec 28. PubMed
PMID: 21189302; PubMed Central PMCID: PMC3024654.

16: Karangelis DE, Kanakis I, Asimakopoulou AP, Karousou E, Passi A, Theocharis
AD, Triposkiadis F, Tsilimingas NB, Karamanos NK. Glycosaminoglycans as key
molecules in atherosclerosis: the role of versican and hyaluronan. Curr Med Chem.
2010;17(33):4018-26. Review. PubMed PMID: 20939824.

17: Vigetti D, Genasetti A, Karousou E, Viola M, Moretto P, Clerici M, Deleonibus
S, De Luca G, Hascall VC, Passi A. Proinflammatory cytokines induce hyaluronan
synthesis and monocyte adhesion in human endothelial cells through hyaluronan
synthase 2 (HAS2) and the nuclear factor-kappaB (NF-kappaB) pathway. J Biol Chem.
2010 Aug 6;285(32):24639-45. doi: 10.1074/jbc.M110.134536. Epub 2010 Jun 3.
PubMed PMID: 20522558; PubMed Central PMCID: PMC2915700.

18: Karousou E, Kamiryo M, Skandalis SS, Ruusala A, Asteriou T, Passi A,
Yamashita H, Hellman U, Heldin CH, Heldin P. The activity of hyaluronan synthase
2 is regulated by dimerization and ubiquitination. J Biol Chem. 2010 Jul
30;285(31):23647-54. doi: 10.1074/jbc.M110.127050. Epub 2010 May 27. PubMed PMID:
20507985; PubMed Central PMCID: PMC2911313.

19: Gualeni B, Facchini M, De Leonardis F, Tenni R, Cetta G, Viola M, Passi A,
Superti-Furga A, Forlino A, Rossi A. Defective proteoglycan sulfation of the
growth plate zones causes reduced chondrocyte proliferation via an altered Indian
hedgehog signalling. Matrix Biol. 2010 Jul;29(6):453-60. doi:
10.1016/j.matbio.2010.05.001. Epub 2010 May 11. PubMed PMID: 20470884.

20: Karousou E, Ronga M, Vigetti D, Barcolli D, Passi A, Maffulli N. Molecular
interactions in extracellular matrix of tendon. Front Biosci (Elite Ed). 2010 Jan
1;2:1-12. Review. PubMed PMID: 20036847.

21: Vigetti D, Genasetti A, Karousou E, Viola M, Clerici M, Bartolini B, Moretto
P, De Luca G, Hascall VC, Passi A. Modulation of hyaluronan synthase activity in
cellular membrane fractions. J Biol Chem. 2009 Oct 30;284(44):30684-94. doi:
10.1074/jbc.M109.040386. Epub 2009 Sep 8. PubMed PMID: 19737932; PubMed Central
PMCID: PMC2781622.

22: Tajana MS, Murena L, Valli F, Passi A, Grassi FA. Correlations between
biochemical markers in the synovial fluid and severity of rotator cuff disease.
Chir Organi Mov. 2009 Apr;93 Suppl 1:S41-8. doi: 10.1007/s12306-009-0004-8.
PubMed PMID: 19711169.

23: Vigetti D, Rizzi M, Viola M, Karousou E, Genasetti A, Clerici M, Bartolini B,
Hascall VC, De Luca G, Passi A. The effects of 4-methylumbelliferone on
hyaluronan synthesis, MMP2 activity, proliferation, and motility of human aortic
smooth muscle cells. Glycobiology. 2009 May;19(5):537-46. doi:
10.1093/glycob/cwp022. Epub 2009 Feb 24. PubMed PMID: 19240269.

24: Cordo-Russo R, Garcia MG, Barrientos G, Orsal AS, Viola M, Moschansky P,
Ringel F, Passi A, Alaniz L, Hajos S, Blois SM. Murine abortion is associated
with enhanced hyaluronan expression and abnormal localization at the fetomaternal
interface. Placenta. 2009 Jan;30(1):88-95. doi: 10.1016/j.placenta.2008.10.013.
Epub 2008 Dec 6. PubMed PMID: 19059644.

25: Vigetti D, Viola M, Karousou E, Genasetti A, Rizzi M, Clerici M, Bartolini B,
Moretto P, De Luca G, Passi A. Vascular pathology and the role of hyaluronan.
ScientificWorldJournal. 2008 Nov 2;8:1116-8. doi: 10.1100/tsw.2008.145. PubMed
PMID: 18979052.

26: Vigetti D, Andrini O, Clerici M, Negrini D, Passi A, Moriondo A. Chondroitin
sulfates act as extracellular gating modifiers on voltage-dependent ion channels.
Cell Physiol Biochem. 2008;22(1-4):137-46. doi: 10.1159/000149791. Epub 2008 Jul
25. PubMed PMID: 18769040.

27: Pirrone F, Mazzola SM, Pastore C, Paltrinieri S, Sironi G, Riccaboni P, Viola
M, Passi A, Clement MG, Albertini M. Activated protein C protection from lung
inflammation in endotoxin-induced injury. Exp Biol Med (Maywood). 2008
Nov;233(11):1462-8. doi: 10.3181/0802-RM-74. Epub 2008 Aug 14. PubMed PMID:
18703750.

28: Vigetti D, Moretto P, Viola M, Genasetti A, Rizzi M, Karousou E, Clerici M,
Bartolini B, Pallotti F, De Luca G, Passi A. Aortic smooth muscle cells migration
and the role of metalloproteinases and hyaluronan. Connect Tissue Res.
2008;49(3):189-92. doi: 10.1080/03008200802143141. PubMed PMID: 18661340.

29: Genasetti A, Vigetti D, Viola M, Karousou E, Moretto P, Rizzi M, Bartolini B,
Clerici M, Pallotti F, De Luca G, Passi A. Hyaluronan and human endothelial cell
behavior. Connect Tissue Res. 2008;49(3):120-3. doi: 10.1080/03008200802148462.
Review. PubMed PMID: 18661325.

30: Viola M, Vigetti D, Genasetti A, Rizzi M, Karousou E, Moretto P, Clerici M,
Bartolini B, Pallotti F, De Luca G, Passi A. Molecular control of the hyaluronan
biosynthesis. Connect Tissue Res. 2008;49(3):111-4. doi:
10.1080/03008200802148405. PubMed PMID: 18661323.

31: Viola M, Vigetti D, Karousou E, Bartolini B, Genasetti A, Rizzi M, Clerici M,
Pallotti F, De Luca G, Passi A. New electrophoretic and chromatographic
techniques for analysis of heparin and heparan sulfate. Electrophoresis. 2008
Aug;29(15):3168-74. doi: 10.1002/elps.200700855. PubMed PMID: 18633938.

32: Karousou E, Ronga M, Vigetti D, Passi A, Maffulli N. Collagens,
proteoglycans, MMP-2, MMP-9 and TIMPs in human achilles tendon rupture. Clin
Orthop Relat Res. 2008 Jul;466(7):1577-82. doi: 10.1007/s11999-008-0255-y. Epub
2008 Apr 19. PubMed PMID: 18425559; PubMed Central PMCID: PMC2505242.

33: Negrini D, Passi A, Moriondo A. The role of proteoglycans in pulmonary edema
development. Intensive Care Med. 2008 Apr;34(4):610-8. doi:
10.1007/s00134-007-0962-y. Epub 2008 Feb 9. Review. PubMed PMID: 18264693.

34: Vigetti D, Viola M, Karousou E, Rizzi M, Moretto P, Genasetti A, Clerici M,
Hascall VC, De Luca G, Passi A. Hyaluronan-CD44-ERK1/2 regulate human aortic
smooth muscle cell motility during aging. J Biol Chem. 2008 Feb
15;283(7):4448-58. Epub 2007 Dec 12. PubMed PMID: 18077444.

35: Genasetti A, Valentino ML, Carelli V, Vigetti D, Viola M, Karousou EG, Melzi
d'Eril GV, De Luca G, Passi A, Pallotti F. Assessing heteroplasmic load in
Leber's hereditary optic neuropathy mutation 3460G->A/MT-ND1 with a real-time PCR
quantitative approach. J Mol Diagn. 2007 Sep;9(4):538-45. Epub 2007 Jul 25.
PubMed PMID: 17652639; PubMed Central PMCID: PMC1975109.

36: Pelosi P, Rocco PR, Negrini D, Passi A. The extracellular matrix of the lung
and its role in edema formation. An Acad Bras Cienc. 2007 Jun;79(2):285-97.
Review. PubMed PMID: 17625682.

37: Moriondo A, Pelosi P, Passi A, Viola M, Marcozzi C, Severgnini P, Ottani V,
Quaranta M, Negrini D. Proteoglycan fragmentation and respiratory mechanics in
mechanically ventilated healthy rats. J Appl Physiol (1985). 2007
Sep;103(3):747-56. Epub 2007 Jun 14. PubMed PMID: 17569774.

38: Negrini D, Passi A. Interstitial matrix and transendothelial fluxes in normal
lung. Respir Physiol Neurobiol. 2007 Dec 15;159(3):301-10. Epub 2007 Apr 8.
Review. PubMed PMID: 17499562.

39: Vigetti D, Moretto P, Viola M, Genasetti A, Rizzi M, Karousou E, Pallotti F,
De Luca G, Passi A. Matrix metalloproteinase 2 and tissue inhibitors of
metalloproteinases regulate human aortic smooth muscle cell migration during in
vitro aging. FASEB J. 2006 Jun;20(8):1118-30. PubMed PMID: 16770011.

40: Mattarucchi E, Marsoni M, Passi A, Lo Curto F, Pasquali F, Porta G.
Establishment and study of different real-time polymerase chain reaction assays
for the quantification of cells with deletions of chromosome 7. J Mol Diagn. 2006
May;8(2):218-24. PubMed PMID: 16645208; PubMed Central PMCID: PMC1867589.

41: Uccella S, Cerutti R, Vigetti D, Furlan D, Oldrini R, Carnevali I, Pelosi G,
La Rosa S, Passi A, Capella C. Histidine decarboxylase, DOPA decarboxylase, and
vesicular monoamine transporter 2 expression in neuroendocrine tumors:
immunohistochemical study and gene expression analysis. J Histochem Cytochem.
2006 Aug;54(8):863-75. Epub 2006 Mar 3. PubMed PMID: 16517981.

42: Vigetti D, Ori M, Viola M, Genasetti A, Karousou E, Rizzi M, Pallotti F,
Nardi I, Hascall VC, De Luca G, Passi A. Molecular cloning and characterization
of UDP-glucose dehydrogenase from the amphibian Xenopus laevis and its
involvement in hyaluronan synthesis. J Biol Chem. 2006 Mar 24;281(12):8254-63.
Epub 2006 Jan 17. PubMed PMID: 16418163.

43: Viola M, Karousou EG, Vigetti D, Genasetti A, Pallotti F, Guidetti GF, Tira
E, De Luca G, Passi A. Decorin from different bovine tissues: study of
glycosaminoglycan chain by PAGEFS. J Pharm Biomed Anal. 2006 Apr 11;41(1):36-42.
Epub 2005 Nov 15. PubMed PMID: 16293388.

44: Negrini D, Tenstad O, Passi A, Wiig H. Differential degradation of matrix
proteoglycans and edema development in rabbit lung. Am J Physiol Lung Cell Mol
Physiol. 2006 Mar;290(3):L470-7. Epub 2005 Oct 7. PubMed PMID: 16214813.

45: Mattarucchi E, Marsoni M, Binelli G, Passi A, Lo Curto F, Pasquali F, Porta
G. Different real time PCR approaches for the fine quantification of SNP's
alleles in DNA pools: assays development, characterization and pre-validation. J
Biochem Mol Biol. 2005 Sep 30;38(5):555-62. PubMed PMID: 16202235.

46: Malek A, Bersinger NA, Di Santo S, Mueller MD, Sager R, Schneider H, Ghezzi
F, Karousou E, Passi A, De Luca G, Raio L. C-reactive protein production in term
human placental tissue. Placenta. 2006 Jun-Jul;27(6-7):619-25. Epub 2005 Jul 18.
PubMed PMID: 16026834.

47: Quaglino D, Sartor L, Garbisa S, Boraldi F, Croce A, Passi A, De Luca G,
Tiozzo R, Pasquali-Ronchetti I. Dermal fibroblasts from pseudoxanthoma elasticum
patients have raised MMP-2 degradative potential. Biochim Biophys Acta. 2005 Jun
30;1741(1-2):42-7. Epub 2004 Oct 8. PubMed PMID: 15955448.

48: Raio L, Cromi A, Ghezzi F, Passi A, Karousou E, Viola M, Vigetti D, De Luca
G, Bolis P. Hyaluronan content of Wharton's jelly in healthy and Down syndrome
fetuses. Matrix Biol. 2005 Apr;24(2):166-74. Epub 2005 Apr 26. PubMed PMID:
15890266.

49: Karousou EG, Viola M, Genasetti A, Vigetti D, Luca GD, Karamanos NK, Passi A.
Application of polyacrylamide gel electrophoresis of fluorophore-labeled
saccharides for analysis of hyaluronan and chondroitin sulfate in human and
animal tissues and cell cultures. Biomed Chromatogr. 2005 Dec;19(10):761-5.
PubMed PMID: 15856490.

50: Karousou EG, Militsopoulou M, Porta G, De Luca G, Hascall VC, Passi A.
Polyacrylamide gel electrophoresis of fluorophore-labeled hyaluronan and
chondroitin sulfate disaccharides: application to the analysis in cells and
tissues. Electrophoresis. 2004 Sep;25(17):2919-25. PubMed PMID: 15349930.

51: Passi A, Sadeghi P, Kawamura H, Anand S, Sato N, White LE, Hascall VC, Maytin
EV. Hyaluronan suppresses epidermal differentiation in organotypic cultures of
rat keratinocytes. Exp Cell Res. 2004 Jun 10;296(2):123-34. PubMed PMID:
15149843.

52: Raio L, Ghezzi F, Cromi A, Cereda E, Passi A. Sonographic morphology and
hyaluronan content of umbilical cords of healthy and Down syndrome fetuses in
early gestation. Early Hum Dev. 2004 Apr;77(1-2):1-12. PubMed PMID: 15113626.

53: Karousou EG, Porta G, De Luca G, Passi A. Analysis of fluorophore-labelled
hyaluronan and chondroitin sulfate disaccharides in biological samples. J Pharm
Biomed Anal. 2004 Mar 1;34(4):791-5. PubMed PMID: 15019056.

54: Vigetti D, Viola M, Gornati R, Ori M, Nardi I, Passi A, De Luca G, Bernardini
G. Molecular cloning, genomic organization and developmental expression of the
Xenopus laevis hyaluronan synthase 3. Matrix Biol. 2003 Nov;22(6):511-7. PubMed
PMID: 14667843.

55: Sciannamblo M, Chigorno V, Passi A, Valaperta R, Zucchi I, Sonnino S. Changes
of the ganglioside pattern and content in human fibroblasts by high density cell
population subculture progression. Glycoconj J. 2002 Mar;19(3):181-6. PubMed
PMID: 12815229.

56: Leoni V, Albertini R, Passi A, Abuja PM, Borroni P, D'Eril GM, De Luca G.
Glucose accelerates copper- and ceruloplasmin-induced oxidation of low-density
lipoprotein and whole serum. Free Radic Res. 2002 May;36(5):521-9. PubMed PMID:
12150540.

57: Miserocchi G, Negrini D, Passi A, De Luca G. Development of lung edema:
interstitial fluid dynamics and molecular structure. News Physiol Sci. 2001
Apr;16:66-71. Review. PubMed PMID: 11390951.

58: Negrini D, Passi A, Bertin K, Bosi F, Wiig H. Isolation of pulmonary
interstitial fluid in rabbits by a modified wick technique. Am J Physiol Lung
Cell Mol Physiol. 2001 May;280(5):L1057-65. PubMed PMID: 11290531.

59: Miserocchi G, Passi A, Negrini D, Del Fabbro M, De Luca G. Pulmonary
interstitial pressure and tissue matrix structure in acute hypoxia. Am J Physiol
Lung Cell Mol Physiol. 2001 May;280(5):L881-7. PubMed PMID: 11290511.

60: Albertini R, Passi A, Abuja PM, De Luca G. The effect of glycosaminoglycans
and proteoglycans on lipid peroxidation. Int J Mol Med. 2000 Aug;6(2):129-36.
Review. PubMed PMID: 10891555.

61: Albertini R, De Luca G, Palladini G, Passi A, Melzi d'Eril GV, Abuja PM.
Mechanistic aspects of the relationship between low-level chemiluminescence and
lipid peroxides in oxidation of low-density lipoprotein. FEBS Lett. 1999 Oct
1;459(1):47-50. PubMed PMID: 10508915.

62: Passi A, Negrini D, Albertini R, Miserocchi G, De Luca G. The sensitivity of
versican from rabbit lung to gelatinase A (MMP-2) and B (MMP-9) and its
involvement in the development of hydraulic lung edema. FEBS Lett. 1999 Jul
30;456(1):93-6. PubMed PMID: 10452537.

63: Miserocchi G, Passi A, Albertini R, Negrini D, De Luca G. Interstitial
pressure and proteoglycan degradation in hydraulic- and elastase-induced lung
edema. Chest. 1999 Jul;116(1 Suppl):31S. PubMed PMID: 10424575.

64: Albertini R, De Luca G, Passi A, Moratti R, Abuja PM. Chondroitin-4-sulfate
protects high-density lipoprotein against copper-dependent oxidation. Arch
Biochem Biophys. 1999 May 1;365(1):143-9. PubMed PMID: 10222048.

65: Passi A, Negrini D, Albertini R, De Luca G, Miserocchi G. Involvement of lung
interstitial proteoglycans in development of hydraulic- and elastase-induced
edema. Am J Physiol. 1998 Sep;275(3 Pt 1):L631-5. PubMed PMID: 9728059.

66: Negrini D, Passi A, De Luca G, Miserochi G. Proteoglycan involvement during
development of lesional pulmonary edema. Am J Physiol. 1998 Feb;274(2 Pt
1):L203-11. PubMed PMID: 9486204.

67: Passi A, Albertini R, Campagnari F, De Luca G. Modifications of proteoglycans
extracted from monolayer cultures of young and senescent human skin fibroblasts.
FEBS Lett. 1997 Dec 29;420(2-3):175-8. PubMed PMID: 9459305.

68: Albertini R, Ramos P, Giessauf A, Passi A, De Luca G, Esterbauer H.
Chondroitin 4-sulphate exhibits inhibitory effect during Cu2+-mediated LDL
oxidation. FEBS Lett. 1997 Feb 17;403(2):154-8. PubMed PMID: 9042957.

69: Passi A, Albertini R, Campagnari F, De Luca G. Modifications of proteoglycans
secreted into the growth medium by young and senescent human skin fibroblasts.
FEBS Lett. 1997 Feb 3;402(2-3):286-90. PubMed PMID: 9037211.

70: Cristallo A, Passi A, Bruseghini L, Cereda PM. Pseudomonas aeruginosa
proteases: purification procedures for an enzymatic standard. New Microbiol. 1996
Jul;19(3):221-6. PubMed PMID: 8841037.

71: Negrini D, Passi A, de Luca G, Miserocchi G. Pulmonary interstitial pressure
and proteoglycans during development of pulmonary edema. Am J Physiol. 1996
Jun;270(6 Pt 2):H2000-7. PubMed PMID: 8764250.

72: Albertini R, Rindi S, Passi A, Pallavicini G, De Luca G. Heparin protection
against Fe2+ -and Cu2+ -mediated oxidation of liposomes. FEBS Lett. 1996 Apr
1;383(3):155-8. PubMed PMID: 8925887.

73: Albertini R, Rindi S, Passi A, Bardoni A, Salvini R, Pallavicini G, De Luca
G. The effect of cornea proteoglycans on liposome peroxidation. Arch Biochem
Biophys. 1996 Mar 15;327(2):209-14. PubMed PMID: 8619604.

74: Albertini R, Rindi S, Passi A, Palladini G, Pallavicini G, De Luca G. The
effect of heparin on Cu(2+)-mediated oxidation of human low-density lipoproteins.
FEBS Lett. 1995 Dec 18;377(2):240-2. PubMed PMID: 8543059.

75: Bardoni A, Salvini R, Pallavicini G, Passi A, Rindi S, Quacci D, Dall'Orbo C,
De Luca G. Collagenase-extractable proteoglycans from lesion-free areas of human
aorta. Ital J Biochem. 1994 Jan-Feb;43(1):1-23. PubMed PMID: 8206740.

76: Passi A, Albertini R, Bardoni A, Rindi S, Salvini R, Pallavicini G, De Luca
G. Modifications of proteoglycans produced by human skin fibroblast cultures
during replicative senescence. Cell Biochem Funct. 1993 Dec;11(4):263-9. PubMed
PMID: 8275551.

77: Pippia P, Formato M, Sciola L, Tilloca G, Bardoni A, Pallavicini G, Passi A,
Rindi S, Salvini R, De Luca G. Modifications of adhesion properties and
proteoglycan structure in rat embryo fibroblast cultures with increasing
passages. Cell Biochem Funct. 1992 Sep;10(3):217-24. PubMed PMID: 1423902.

78: Balduini C, De Luca G, Passi A, Rindi S, Salvini R, Scott JE. Effect of
oxygen tension and lactate concentration on keratan sulphate and chondroitin
sulphate biosynthesis in bovine cornea. Biochim Biophys Acta. 1992 Jan
23;1115(3):187-91. PubMed PMID: 1739733.

79: Balduini C, De Luca G, Passi A, Rindi S, Salvini R, Scott JE. Differential
effect of oxygen supply and lactate concentration on keratan sulphate and
glycosaminoglycuronan production in bovine cornea. Biochem Soc Trans. 1991
Nov;19(4):354S. PubMed PMID: 1794492.